Indian Council of Agricultural Research
CENTRAL MARINE FISHERIES RESEARCH INSTITUTE

HomeScientific Staff Dr.M. A. Pradeep

Dr.M. A. Pradeep

Tel: +91 484 2331155; Cell: 
Email: drpradeepma@gmail.com,  
Role:Scientist
Focus of Research:
  • Molecular Diagnostics and recombinant and DNA Vaccine Technologies
  • Functional  genomics and proteomics: focus on reproduction, disease and stress
  • Transgenic animal models 
Background:

After obtaining Bachelors in Veterinary Science and Animal Husbandry from College of Veterinary and Animal Sciences, Mannuthy , Kerala, I took Masters in Veterinary Science (Animal Biotechnology) from National Dairy research Institute, Karnal, Haryana under the mentorship of Dr A.K. Mohanty, the research focus during my masters was on oviduct specific glycoproteins (Oviductin) from ruminants( goat and buffalo), Oviductin gene was characterized and native oviductin protein purified to established its significance during fertilisation via IVF experiments.  After which I obtained my PhD from the same institute under the mentorship of Dr. J.K. Kaushik, the area of my research was on different types of lysozymes from ruminants (buffalo, cow and Sheep) wherein mammarygland, abomasal and testis specific lysozyme genes were characterisaed and expressed recombinantly and purified, we also established the significance of testis specific lysozymes and its role in sperm egg binding and fertilization.

 I joined CMFRI as a scientist on March 2010. My area of work is functional proteomics and genomics, one of our focus areas is molecular mechanisms associated with reproduction in crustaceans and fin fishes, The Gonad Inhibiting Hormone (GIH) from Penaes monodon has been cloned and characterized, soluble recombinant GIH has been expressed in prokaryotic expression system for the first time.

Our lab also works on diseases diagnostics and health management, where we research and develop molecular and immune-diagnostics (Q-PCR, Nested- PCR, LAMP) and vaccines (recombinant and DNA).  Transgenic animal model is another area of interest in the lab.

In the recently completed NAIP funded project on “Bioprospecting of genes and allele mining for abiotic stress tolerance” we have identified and functionally validated stress tolerant genes from specific stress tolerant microalgae isolated from Salt pans, hot springs and acidic environments. 

Education:
  • PhD  2011  National Dairy Research Institute, Karnal, Haryana
  • MVSc  2005  National Dairy Research Institute, Karnal, Haryana
  • BVSc  2002  College of Veterinary and Animal Sciences, Mannuthy, Kerala   
Current Research Projects
No Title Funded By
1 Genetics, genomics and biotechnological applications in mariculture and fishery resources management CMFRI
2 Health Management in selected finfish and shellfish for mariculture and aquariculture & bioprospecting from marine resources CMFRI
3 National Surveillance Programme for Aquatic Animal Diseases NFDB
4 Mapping of deep-sea and hypersaline microbiota and their
characterization using meta-omics approaches for bioprospecting novel genes
AMAAS
Awards & Recognitions:
  • CSIR (JRF, SRF) fellowship for PhD
  • ICAR institutional fellowship for MVSc
  • Best poster award, 2006 (International conference, ISSRF)
  • Best poster award, 2007 (International conference, ISSRF)
Publications:

eprints@cmfri

Best 5 Publications:

1. Gyanaranjan Dash, Ram Prakash Raman, K. Pani Prasad, M. Makesh, Pradeep M.A. (2014). Evaluation of Lactobacillus plantarum as feed supplement on host associated microflora, growth, feed efficiency, carcass biochemical composition and immune response of giant freshwater prawn, Macrobrachium rosenbergii (de Man, 1879). Aquaculture. (accepted)
2. Anusree V Nair, Pradeep M A, Vijayan K K (2014). Molecular approach for the rapid detection of Bacillus and Pseudomonas genera-dominant antagonistic groups-from diverse ecological niches using colony multiplex PCR. Journal of Industrial Microbiology and Biotechnology.
3. Janjanam J, Singh S, Choudhary S, Pradeep MA, Kumar S, Kumaresan A, Das SK, Kaushik JK, Mohanty AK. (2012). Molecular cloning, sequence characterization and heterologous expression of buffalo (Bubalus bubalis) oviduct-specific glycoprotein in E. coli. Mol Biol Rep. 39: 10031-10043
4. Pradeep, M. A., Jagadeesh, J., De, A. K., Kaushik, J. K., Malakar, D., Kumar, S., Dang, A. K., Das, S. K., Mohanty, A. K. (2011). Purification, sequence characterization and effect of goat oviduct-specific glycoprotein on in vitro embryo development. Theriogenology, 75: 213-218
5. Mohanty, A. K., Fisher, A. J., Yu, Z., Pradeep, M. A., Janjanam, J., Kaushik, J. K. (2009). Cloning, expression, characterization and crystallization of BRP39, a signalling glycoprotein expressed during mammary gland apoptosis. Prot Exp Purif. 64: 213–218

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